Label-free microscopy
with high image
quality
Live-cell imaging allows researchers to determine not only whether, but also how and when certain biological events occur in cell culture. In order to achieve the high image quality standards required for data analysis and publications, live-cell imaging is performed predominantly using a microscope with a stage top incubation box. However, the culture conditions in the incubation box are more prone to fluctuations, as compared to a dedicated cell culture incubator. Using the CytoSMART Lux3 BR, it is no longer necessary to choose between optimal cell culture conditions and image quality.
Live-cell imaging has become increasingly valuable in the fields of cancer research, drug discovery, immunology, tissue engineering, stem cell research, and 3D cell culture models. Various experimental applications, including cell viability and cell differentiation monitoring, spheroid and organoid characterization, microfluidic chip platforms, cell morphology analysis, single-cell tracking, and angiogenesis monitoring can benefit from detailed kinetic visualization.
Incubator-friendly live-cell imaging
The cell culture environment inside a stage top incubation box is generally sub-optimal for maintaining live cells. The CytoSMART Lux3 BR is designed to work inside a standard CO2 incubator, without disturbing the internal airflow and temperature. This allows you to perform long-term cell imaging experiments at the optimum culture conditions.
Digital images suitable for scientific publications
The CytoSMART Lux3 BR is ideal for capturing crisp brightfield images and videos of living cells. The image size of 2072×2072 pixels combined with the 1.45×1.45 mm field of view provide a resolution of 0.7 µm/pixel. Even at the commonly required image resolution of 300 dpi for printed scientific publications, these images can fill the entire page width if desired, without compromising the image quality.
Remote and automated analysis of cell cultures
Images and videos of running or completed experiments can be accessed, processed, and analyzed from any desired location using the CytoSMART Cloud-based environment. Therefore, cells can be monitored without having to open the incubator, or even be in the lab. The integrated cloud-based image analysis facilitates quantification of output parameters, e.g. cell confluence or wound healing rate. The automated quantification minimizes avoidable variation and bias in the interpretation of results.
Easy to expand to a multiple-device setup
A setup with the CytoSMART Lux3 BR can be easily expanded to two or even four devices that can be operated and controlled individually via a single laptop. Since all devices of the CytoSMART Lux3 BR Duo or Multi Lux3 BR Kits can be placed directly next to each other in the same incubator, the monitored cultures are maintained in an identical culture environment. This facilitates optimal simultaneous comparison of experimental groups, with minimal variations in environmental factors, but also represents a convenient solution for large research groups.
Technical specifications
| Channel | Brightfield with digital phase contrast |
|---|---|
| Camera | 6.4 MP CMOS |
| Magnification | 10× fixed objective – 20× digital zoom |
| Image resolution | 2072 × 2072 pixels |
| Field of view | 1.45 × 1.45 mm |
| Light source | LED |
| Data formats | JPG, TIFF, XLSX, MP4 |
| Data storage | Unlimited cloud storage |
| Computer requirements | Windows 10, USB 3.0 |
| Dimensions (L × W × H) | 16.6 cm × 14.0 cm × 13.5 cm |
| Weight | 1.3 kg |
| Culture vessels | Well-plates, petri dishes, flasks, microfluidic chips, and custom culture vessels |
| Operating conditions | 5 – 40°C, 20 – 95% humidity |
| Warranty | 1-year parts and labor |
| Extra features | Confluence analysis algorithm, scratch assay analysis algorithm |
| Research use only. Not intended for diagnostic purposes |
Videos
Downloads
Cyto SMART Lux3 BR product features 1 pager PDF
CytoSMART-Lux3-BR-product-features-1-pager-PDF ( PDF - 2 MB )
Cyto SMART Lux3 BR product features 2 pager PDF
CytoSMART-Lux3-BR-product-features-2-pager-PDF ( PDF - 5 MB )
Lux3 BR Brochure PDF
Lux3-BR-Brochure-PDF ( PDF - 12 MB )
App Note Accurate tracking of chemotactic cancer cell migration - basic insights in metastasis
APPnote4001-Lux3-BR_2022-02-08-095044_otjh ( PDF - 4 MB )
Frequently Asked Questions
The CytoSMART Lux3 BR is an inverted digital microscope that utilizes brightfield and digital phase-contrast microscopy to capture high-quality images of living cells. Due to its compact size, the microscope can be placed directly inside a standard cell culture incubator, allowing to perform long-term imaging experiments without sacrificing cell health and viability.
The applications of the Lux3 BR include but not limited to – monitoring cell viability and cell differentiation, spheroid and organoid characterization, cell morphology analysis, and single-cell tracking. Researchers in the fields of drug discovery, immunology, tissue engineering, cancer and stem cell research can benefit from live-cell imaging.
The CytoSMART BR comes with a fixed 10× objective and 20× digital zoom.
Cell confluence, scratch analysis, and colony detection are currently a part of the image analysis software package. Users always have the option to download raw image data and perform their own analysis.
No, our image analysis algorithms are optimized to be used in label-free screens and assays, so you don’t have to add any fluorescent or colorimetric dyes to your culture, providing a non-invasive analysis of the cells.
The CytoSMART Lux3 BR allows to monitor a wide range of different culture dishes and flasks, including:
- T-flasks: T-25 up to T-250
- Single-well, multi-well plates (6 – 384-well plates)
- Microfluidic chips
- Cell culture tubes
- Petri dishes
- Slides